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Reagents kits intended for detecting M class antibodies to German measles virus, herpes and toxoplasmosis using “capture” method.

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  • Reagents kit German measles-IgМ-”capture” for immunoenzyme detection of M class antibodies to German measles virus;
  • Reagents kit HSV-IgM-“capture” for immunoenzyme detection of M class antibodies to herpes simplex virus of types 1 and 2;
  • Reagents kit Toxo-IgM-“capture” for immunoenzyme detection of M class antibodies to Toxoplasma gondii.

New test-systems for detecting M class antibodies involve capture option of ELISA with fixed antibodies or “antibody capture ELISA”. The method is based on the following: affinely purified antibodies specifically reacting with human IgM class antibodies are fixed on hardphase carrier. As a result of incubation with tested serum antibodies of this class connect with carrier. The presence of antibodies among them specific for any antigen is determined during the incubation with this antigen tagged with enzyme. Solution dyeing is observed by incubation with a substrate in case if there were specific antibodies in test specimen.

Applying capture option of ELISA lead to increase of susceptibility of analyze in case of synchronous presence of M and G class immunoglobulins in a test sample. A specific interaction of serum IgM with anti-IgM-antibodies sorbed on the plate holes surface takes place on the first step of the test option suggested; IgG remained in serum tested are removed by washing. On the step 2 the specific IgМ are revealed from all fixed M class immunoglobulins by making them interacted with specific conjugate. Conventional ELISA option means that both IgM and IgG bound with fixed antigens on the plate surface, competing for the binding sites. Test susceptibility of this ELISA option depends on the correlation of specific IgM and IgG concentrations in a sample tested.

Whereas using ELISA capture option allows avoiding false positive reactions relating to blood rheumatoid factor, cross-reactions between М and G immunoglobulins.

Using specifically selected recombinant antigens for conjugate allows avoiding false positive results relating to cross-reactions between causative reagents and other allied microorganisms.

Thereby the presence of specific IgG-antibodies, rheumatoid factor and antinuclear antibodies in serum potentially does not influence reaction results and makes test-kits intended for M antibodies detection more susceptible and specific.